What is the Biuret Test for Proteins? The Biuret Test is often used to determine the presence of peptide bonds in protein. As a student you will most likely will be testing for the presence of protein in foods. The Biuret test for proteins may also be extended to quantitatively measure the concentration of total protein using spectrometric methods. The Biuret reagent is the sole reagent in the Biuret Test for proteins. The Biuret reagent contains Hydrated Copper sulphate Potassium hydroxide solution Potassium sodium tartrate Are the alternative reagents for the Biuret Test for Proteins? As an alternative to the Biuret Reagent , either of the following solutions may be used to yield comparable results. Sodium Hydroxide and Copper Sulphate Solutions Fehling’s Solutions A and B PROCEDURE Three procedures are outlined below using different reagents. For each of the procedures, a liquid sample must be prepared from solid foods as follows. PREPAR
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